Document Type : Review Article


Department of Aesthetic / College of Health and Medical Techniques/ University of Al Zahraa for woman, Karbala, Iraq.


Chronic B-cell neoplasms include a number of disease entities arising from mature B lymphocytes which involve primarily the blood, bone marrow (BM) and lymphoid organs such as the lymph nodes and spleen. Chronic lymphocytic leukemia (CLL) is characterized by absolute lymphocytosis in the peripheral blood and BM. Non-Hodgkin lymphomas (NHL) also may have extensive BM and peripheral blood involvement at initial presentation like follicular lymphoma (FL), mantle cell lymphoma (MCL) and splenic marginal zone lymphoma (SMZL). 
      Although there are difficulties in separating CLL from some NHL, the distinction is important because prognostic and therapeutic differences exist. Immunophenotyping (IPT) has become an essential tool to confirm the diagnosis and to separate CLL from other lymphoid malignancies. Evaluate the role of immunostained CD10 and CD20 in the subclassification of chronic B-cell neoplasms and confirm the significance of these markers as a complementary test to morphological features of chronic B-cell neoplasms using BM aspirate and biopsy. BM biopsies of fifty five adult patients with CLL and leukemic phase of NHL were collected from December 2010 to April 2011; fifty of them were retrospectively while five of them were prospectively collected. Current study revealed that CLL cases (27) showed weak positive reaction with CD20 (96%) while no reaction with CD10 (0%). While NHL (28) cases showed positive reaction with CD20 (100%) &  CD10 (71.5%). This study revealed significant statistical difference between morphology and IPT diagnosis at the level of P.value <0.05.
This study revealed that the CD markers has important diagnostic role in the subclassification of chronic B-cell neoplasms also revealed that the IPT technique in conjunction with morphology have more precise role than morphology alone in the diagnosis of chronic B-cell neoplasms.